An ATP-selective, lanthanide complex luminescent probe.

نویسندگان

  • Xiao Liu
  • Jun Xu
  • Yinyun Lv
  • Wenyu Wu
  • Weisheng Liu
  • Yu Tang
چکیده

A luminescent probe based on a europium complex is developed, which effectively distinguishes adenosine-5'-triphosphate (ATP) from adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in pure water at pH 6.8. With a longer lifetime (in ms range), the probe is prospectively applied to biological systems to monitor ATP levels by completely removing the background fluorescence of other molecules.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A long-lived luminescence and EPR bimodal lanthanide-based probe for free radicals.

We developed a novel spin-labeled terbium complex Tb(3+)/cs124-DTPA-TEMPO (1) by covalently labeling a nitroxide radical on the terbium complex for monitoring free radicals of various areas. This lanthanide complex probe shows a high EPR signal which resulted from the nitroxide radical moiety, and is weakly luminescent which resulted from the intramolecular quenching effect of the nitroxide rad...

متن کامل

Development of a zinc ion-selective luminescent lanthanide chemosensor for biological applications.

Detection of chelatable zinc (Zn(2+)) in biological studies has attracted much attention recently, because chelatable Zn(2+) plays important roles in many biological systems. Lanthanide complexes (Eu(3+), Tb(3+), etc.) have excellent spectroscopic properties for biological applications, such as long luminescence lifetimes of the order of milliseconds, a large Stoke's shift of >200 nm, and high ...

متن کامل

A Label-Free Luminescent Switch-On Assay for ATP Using a G-Quadruplex-Selective Iridium(III) Complex

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of...

متن کامل

Turning an Aptamer into a Light-Switch Probe with a Single Bioconjugation

We describe a method for transforming a structure-switching aptamer into a luminescent light-switch probe via a single conjugation. The methodology is demonstrated using a known aptamer for Hg(2+) as a case study. This approach utilizes a lanthanide-based metallointercalator, Eu-DOTA-Phen, whose luminescence is quenched almost entirely and selectively by purines, but not at all by pyrimidines. ...

متن کامل

An amine/imine functionalized microporous MOF as a new fluorescent probe exhibiting selective sensing of Fe3+ and Al3+ over mixed metal ions

Nowadays metal-organic frameworks with multiple luminescent centers are very fascinating as multifunctional luminescent material because of their luminescence properties, which could be systematically tuned by deliberate use of organic ligands and metal ions. In this research, we explored a microporous mixed-ligand MOF for highly selective and sensitive detection of metal ions. A two-fold inter...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Dalton transactions

دوره 42 27  شماره 

صفحات  -

تاریخ انتشار 2013